Bacteria



BCRC Number: 14612
Organism: Bifidobacterium breve
Author: Reuter
History: << NCDO (Bifidobacterium bifidum) << NCIMB
Other Collection No.: NCDO 1456; NCIMB 8815
Source: nursling stools
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
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References



 

Medium



252
LACTOBACILLI MRS BROTH with 0.05% Cysteine
Ammonium citrate2.0g
Beef extract10.0g
CH3COONa5.0g
Cysteine0.5g
Dextrose20.0g
Distilled water1.0L
K2HPO42.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
Proteose peptone No.310.0g
Tween 801.0g
Yeast extract5.0g
1.After preparation, distribute tubes under N2 and autoclave.
2.If needed, add agar powder 15.0 gram per liter.

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