Bacteria



BCRC Number: 14551 Add To Bookmark
Organism: Clostridium scindens
Author: Morris et al.
History: << ATCC << L. V. Holdeman, VPI 13733 << V. Bokkenheuser 19
Other Collection No.: ATCC 35704 ;DSM 5676
Source: human feces, New York
Characterization:
Production : steroid desmolase, 7a -dehydroxylase, 7b -dehydrogenase, 20-a -hydroxysteroid dehydrogenase--B1831, B1833;
Category: anaerobe
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1831
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References



B1831
Morris, G. N., J. Winter, E. P. Cato, A. E. Ritchie, and V. D. Bokkenheuser. 1985. Clostridium scindens sp. nov., a human intestinal bacterium with desmolytic activity on corticoids. Int. J. Syst. Bacteriol. 37: 478-481.
B1833
Krafft, A. E., and P. B. Hylemon. 1989. Purification and characterization of a novel form of 20-hydroxysteroid dehydrogenase from Clostridium scindens. J. Bacteriol. 171: 2925-2932.

Medium



72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
859
Chocolate II Agar (BBL)
Pancreatic Digest of Casein7.5 g
Selected Meat Peptone7.5 g
Corn Starch1.0 g
Dipotassium Phosphate4.0 g
Monopotassium Phosphate1.0 g
Sodium Chloride5.0 g
Hemoglobin10.0 g
IsoVitaleX Enrichment10.0 mL
Agar12.0 g
Distilled water1000 mL
IsoVitaleXTM Enrichment
Vitamin B120.01 g
L-Glutamine10.0 g
Adenine0.03 g
/p/-Aminobenzoic Acid0.013 g
Nicotinamide Adenine Dinucleotide0.25 g
Thiamine Pyrophosphate0.1 g
Ferric Nitrate0.02 g
Thiamine Hydrochloride0.003 g
L-Cysteine Hydrochloride25.9 g
L-Cystine1.1 g
Dextrose100.0 g
Distilled water1000 mL

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