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Bacteria



BCRC Number: 14548 Add To Bookmark
Organism: Paraclostridium ghonii
Synonym: Clostridium ghonii
Author: (Prévot) Bello et al.
History: << ATCC << L. DS. Smith, VPI 4897 << A. Prevot strain PEV
Other Collection No.: ATCC 25757 ;JCM 1400 ;VPI 4897 ;DSM 15049
Characterization:
Taxonomy : Rename Clostridium to Paraclostridium.--B000224,B000387;
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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References



B000224
Sasi, J. T. S., L. Tushar, C. Sasikala, and C. V. Ramana. 2016. Paraclostridium benzoelyticum gen. nov., sp. nov., isolated from marine sediment and reclassification of Clostridium bifermentans as Paraclostridium bifermentans comb. nov. Proposal of a new genus Paeniclostridium gen. nov. to accommodate Clostridium sordellii and Clostridium ghonii. Int. J. Syst. Evol. Microbiol. 66: 1268-1274.
B000387
Bello, S., S. McQuay, B. Rudra, and R. S. Gupta. 2024. Robust demarcation of the family Peptostreptococcaceae and its main genera based on phylogenomic studies and taxon-specific molecular markers. Int. J. Syst. Evol. Microbiol. 74: 6247.
B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.

Medium



229
MODIFIED CHOPPED MEAT MEDIUM
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Agar (if necessary)20.0 g
Cysteine·HCl0.5 g
Distilled water1.0 L
Ground beef (fat free)500.0 g
Hemin solution (see below)10.0 mL
K2HPO45.0 g
Trypticase peptone30.0 g
Vitamin K1 solution (see below)0.2 mL
Yeast extract5.0 g
Mix meat, water and NaOH and bring to a boil with stirring. Cool to room temperature, skim fat from surface, and filter, retaining both meat particles and filtrate. To filtrate, add sufficient distilled water to restore volumn to 1.0 L. To this filtrate, add trypticase peptone, yeast extract, K2HPO4, resazurin solution and agar (if necessary). Boil and cool medium under 80% N2, 10% CO2, and 10% H2, then add Cysteine-HCl, Hemin solution and Vitamin K1 solution. Adjust pH to 7.0. Anaerobically dispense 7 mL into tubes containing meat particles (1 part meat to 5 parts fluid) under the same gas phase.
Hemin solution:
Dissolve 50 mg hemin in 1mL 1N NaOH, make up to 100 mL with distilled water. Store refrigerated.

Vitamin K1 solution:
Dissolve 0.15 mL of Vitamin K1 in 30 mL of 95% ethanol and filter sterile. Keep Vitamin K1 solution under refrigeration. Store in brown bottle.

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