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Bacteria



BCRC Number: 14545
Organism: Clostridium clostridioforme
Author: (Burri and Ankersmit) Kaneuchi et al.
History: << ATCC << L. V. Holdeman, VPI 0316 << M. P. Bryant
Other Collection No.: ATCC 25537; DSM 933
Source: calf rumen
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1803
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B1803
Kaneuchi, C., K. Watanabe, A. Terada, Y. Benno, and T. Mitsuoka. 1976. Taxonomic study of Bacteroides clostridiiformis subsp. clostridiiformis (Burriand Ankersmit) Holdeman and Moore and of related organisms: proposal of Clostridium clostridiiformis (Burri and Ankersmit) comb. nov. and Clostridium sym
 

Medium



321
CHOPPED MEAT MEDIUM with Carbohydrates II
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Cellobiose1.0 g
Distilled water1.0 L
Ground beef (fat free)500.0 g
K2HPO45.0 g
L-cysteine·HCl0.5 g
Maltose1.0 g
Peptone30.0 g
Starch1.0 g
Yeast extract5.0 g
53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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