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BCRC Number: 14540 Add To Bookmark
Organism: Clostridium scatologenes
Author: (Weinberg and Ginsbourg) Prevot
History: << DSMZ << NCIMB << H. Rosenberger, VPI 5393
Other Collection No.: ATCC 25775 ;DSM 757 ;NCIMB 8855
Category: anaerobe
Growth Conditions: 37°C 4 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1823
Freeze-Dried for all NTD$ 5000 Order this item


Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
Johnson, J. L., and B. S. Francis. 1975. Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species. J. Gen. Microbiol. 88: 229-244.


CHOPPED MEAT MEDIUM with Carbohydrates I
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Cellobiose1.0 g
Distilled water1.0 L
Glucose4.0 g
Ground beef (fat free)500.0 g
K2HPO45.0 g
L-cysteine·HCl0.5 g
Maltose1.0 g
Peptone30.0 g
Starch1.0 g
Yeast extract5.0 g
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust. Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch before adding cysteine.
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
Chocolate II Agar (BBL)
Pancreatic Digest of Casein7.5 g
Selected Meat Peptone7.5 g
Corn Starch1.0 g
Dipotassium Phosphate4.0 g
Monopotassium Phosphate1.0 g
Sodium Chloride5.0 g
Hemoglobin10.0 g
IsoVitaleX Enrichment10.0 mL
Agar12.0 g
Distilled water1000 mL
IsoVitaleXTM Enrichment
Vitamin B120.01 g
L-Glutamine10.0 g
Adenine0.03 g
/p/-Aminobenzoic Acid0.013 g
Nicotinamide Adenine Dinucleotide0.25 g
Thiamine Pyrophosphate0.1 g
Ferric Nitrate0.02 g
Thiamine Hydrochloride0.003 g
L-Cysteine Hydrochloride25.9 g
L-Cystine1.1 g
Dextrose100.0 g
Distilled water1000 mL


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