Bacteria



BCRC Number: 14534 Add To Bookmark
Organism: Clostridium tetanomorphum
Author: Wilde et al.
History: << DSMZ << NCTC << M. Robertson, 259E, III
Other Collection No.: DSM 4474 ;NCTC 543;ATCC 49273
Source: septic wound--B1818
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1819,B1820
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References



B1819
Wilde, E., H. Hippe, N. Tosunoglu, G. Schallehn, K. Herwig, and G. Gottschalk. 1989. Clostridium tetanomorphum sp. nov. nom. rev. Int. J. Syst. Bacteriol. 39: 127-134.
B1820
Nakamura, S.,I. Okado, T. Abe, and S. Nishida. 1979. Taxonomy of Clostridium tetani and related species. J. Gen. Microbiol. 113: 29-35.

Medium



1036
CDC anaerobic Blood Agar
Pancreatic Digest of Casein15.0 g
Soy peptone5.0 g
NaCl5.0 g
Yeast extract5.0 g
L-Cystine0.4 g
NaOH4.0 mL
Hemin(4.0% solution)5.0 mL
Vitamin K1(0.1% solution)1.0 mL
Agar15.0 g
Distilled water1.0 L
Defibrinated sheep blood50.0 mL
After autoclaved, when the medium cooling down to room temperature 45 degree celsius, added the defibrinated sheep blood.
191
CHOPPED MEAT MEDIUM with Carbohydrates I
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Cellobiose1.0 g
Distilled water1.0 L
Glucose4.0 g
Ground beef (fat free)500.0 g
K2HPO45.0 g
L-cysteine·HCl0.5 g
Maltose1.0 g
Peptone30.0 g
Starch1.0 g
Yeast extract5.0 g
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust. Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch before adding cysteine.
320
WILKINS-CHALGREN ANAEROBE BROTH (OXOID CM643)
Supplement with 1 mg resazurin and 0.3 g L-cysteine per liter.

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