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Bacteria



BCRC Number: 14529 Add To Bookmark
Organism: Clostridium oroticum
Author: (Wachsman and Barker) Cato et al.
History: << ATCC << H. C. Friedmann << A. Kornberg
Other Collection No.: ATCC 13619 ;ATCC 25750 ;DSM 1287 ;NCIMB 10650 ;KCTC 15331
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1815,B1816
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B1815
Cato, E. P.,W. E. C. Moore, and L. V. Holdeman. 1968. Clostridium oroticum comb. nov. emended description. Int. J. Syst. Bacteriol. 18: 9-13.
B1816
Wachsman, J. T., and H. A. Barker. 1954. Characterization of an orotic acid fermenting bacterium, Zymobacterium oroticum, nov. gen., nov. spec. J. Bacteriol. 68: 400-404.

Medium



319
ZYMOBACTERIUM MEDIUM
NaOH0.5 g
Na3PO4·12H2O1.5 g
Na-Thioglycollate1.0 g
Riboflavin0.015 g
Agar (if needed)15.0 g
Tryptone20.0 g
Orotic acid2.0 g
Distilled water1.0 L
Add ingredients in the order listed at room temperature. Orotic acid will not dissolve completely. The final pH should be 7.9. If it is found to be higher, add a small amount of NaH2PO4. Agitate the mixture each time prior to dispens

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