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BCRC Number: 14528 Add To Bookmark
Organism: Clostridium irregulare
Synonym: Clostridium irregularis
Author: (Choukevitch) Prevot
History: << DSM << ATCC << L. DS. Smith, VPI 4428 << A. R. Prevot, 6VI
Other Collection No.: ATCC 25756 ;DSM 2635 ;KCTC 15330
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1813,B1814
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Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
Prevot, A. R. 1938. Etudes de systematique bacterienne IV. Critique de la conception actuelle du genre Clostridium. Ann. Microbiol. (Inst. Pasteur) 61: 72-91.
Holdeman, L. V., E. P. Cato, and W. E. C. Moore. 1977. Clostridium. Anaerobe Laboratory Manual, 4th ed. p. 79-106. Virginia Polytechn. Inst. and State Univ., Blacksburg, Virginia, U. S. A.


0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Agar (if necessary)20.0 g
Cysteine·HCl0.5 g
Distilled water1.0 L
Ground beef (fat free)500.0 g
Hemin solution (see below)10.0 mL
K2HPO45.0 g
Trypticase peptone30.0 g
Vitamin K1 solution (see below)0.2 mL
Yeast extract5.0 g
Mix meat, water and NaOH and bring to a boil with stirring. Cool to room temperature, skim fat from surface, and filter, retaining both meat particles and filtrate. To filtrate, add sufficient distilled water to restore volumn to 1.0 L. To this filtrate, add trypticase peptone, yeast extract, K2HPO4, resazurin solution and agar (if necessary). Boil and cool medium under 80% N2, 10% CO2, and 10% H2, then add Cysteine-HCl, Hemin solution and Vitamin K1 solution. Adjust pH to 7.0. Anaerobically dispense 7 mL into tubes containing meat particles (1 part meat to 5 parts fluid) under the same gas phase.
Hemin solution:
Dissolve 50 mg hemin in 1mL 1N NaOH, make up to 100 mL with distilled water. Store refrigerated.

Vitamin K1 solution:
Dissolve 0.15 mL of Vitamin K1 in 30 mL of 95% ethanol and filter sterile. Keep Vitamin K1 solution under refrigeration. Store in brown bottle.


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