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Bacteria



BCRC Number: 14527 Add To Bookmark
Organism: Clostridium sporosphaeroides
Synonym: Clostridium sporosphaeroides
Author: Rios-Galicia et al.
History: << DSMZ << ATCC << L. DS. Smith, VPI 4527
Other Collection No.: ATCC 25781 ;DSM 1294 ;NCIMB 10672
Source: infected wound
Characterization:
Taxonomy : --B000395;
Others: 1.Rename as Ref. no. B000395.
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1823,B000395
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References



B000395
Rios-Galicia, B., J. S. Saenz, T. Yergaliyev, C. Roth, A. Camarinha-Silva, and J. Seifert. 2024. Novel taxonomic and functional diversity of eight bacteria from the upper digestive tract of chicken. Int J Syst Evol Microbiol 74: 6210.
Keyword
Lactobacillaceae; chicken; crop; culture collection; ileum; jejunum; microbiome; novel taxa
B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B1823
Johnson, J. L., and B. S. Francis. 1975. Taxonomy of the clostridia: ribosomal ribonucleic acid homologies among the species. J. Gen. Microbiol. 88: 229-244.

Medium



229
MODIFIED CHOPPED MEAT MEDIUM
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Agar (if necessary)20.0 g
Cysteine·HCl0.5 g
Distilled water1.0 L
Ground beef (fat free)500.0 g
Hemin solution (see below)10.0 mL
K2HPO45.0 g
Trypticase peptone30.0 g
Vitamin K1 solution (see below)0.2 mL
Yeast extract5.0 g
Mix meat, water and NaOH and bring to a boil with stirring. Cool to room temperature, skim fat from surface, and filter, retaining both meat particles and filtrate. To filtrate, add sufficient distilled water to restore volumn to 1.0 L. To this filtrate, add trypticase peptone, yeast extract, K2HPO4, resazurin solution and agar (if necessary). Boil and cool medium under 80% N2, 10% CO2, and 10% H2, then add Cysteine-HCl, Hemin solution and Vitamin K1 solution. Adjust pH to 7.0. Anaerobically dispense 7 mL into tubes containing meat particles (1 part meat to 5 parts fluid) under the same gas phase.
Hemin solution:
Dissolve 50 mg hemin in 1mL 1N NaOH, make up to 100 mL with distilled water. Store refrigerated.

Vitamin K1 solution:
Dissolve 0.15 mL of Vitamin K1 in 30 mL of 95% ethanol and filter sterile. Keep Vitamin K1 solution under refrigeration. Store in brown bottle.

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