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BCRC Number: 14523 Add To Bookmark
Organism: Clostridium nexile
Author: Holdeman and Moore
History: << DSMZ << E. P. Cato << L. V. Holdeman and W. E. C. Moore, VPI C48-37
Other Collection No.: ATCC 27757 ;DSM 1787
Source: normal human faeces--B1810
Category: anaerobe
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1810
Freeze-Dried for all NTD$ 5000 Order this item


Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
Holdeman, L. V., and W. E. C. Moore. 1974. New genus, Coprococcus, twelve new species, and emended descriptions of four previously described species of bacteria from human feces. Int. J. Syst. Bacteriol. 24: 260-277.


CHOPPED MEAT MEDIUM with Carbohydrates I
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Cellobiose1.0 g
Distilled water1.0 L
Glucose4.0 g
Ground beef (fat free)500.0 g
K2HPO45.0 g
L-cysteine·HCl0.5 g
Maltose1.0 g
Peptone30.0 g
Starch1.0 g
Yeast extract5.0 g
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust. Add 0.4% glucose, 0.1% cellobiose, 0.1% maltose, and 0.1% soluble starch before adding cysteine.
GAM Broth, Modified
Peptone 5.0 g
Soya Peptone 3.0 g
Proteose Peptone 5.0 g
Digested Serum 10.0 g
Yeast Extract 2.5 g
Meat Extract 2.2 g
Liver Extract 1.2 g
Dextrose0.5 g
Soluble Starch 5.0 g
L-Tryptophan 0.2 g
L-Cysteine Hydrochloride 0.3 g
Sodium Thioglycollate 0.3 g
L-Arginine 1.0 g
Vitamin K15 mg
Hemin10 mg
Potassium Dihydrogen Phosphate 2.5 g
Sodium Chloride3.0 g
pH 7.1


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