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Bacteria



BCRC Number: 14517
Organism: Clostridium innocuum
Author: Smith and King
History: << ATCC << L. DS. Smith B-3 << CDC (E. King) << Murfreesboro Med. Clinic, TN.
Other Collection No.: ATCC 14501 ;DSM 1286 ;NCIMB 10674
Source: appendiceal abscess
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1806
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B1806
Smith L. DS., and E. King. 1962. Clostridium innocuum, sp. n., a spore-forming anaerobe isolated from human infections. J. Bacteriol. 83: 938-939.
 

Medium



167
BHI BROTH with CaCO3
Brain Heart Infusion Broth (dehydrated)37.0 g
Distilled water1.0 L
Cysteine HCl·H2O0.5 g
Hemin solution (see medium 199)10.0 ml
Vitamin K1 (see medium 199)0.2 g
Yeast extract5.0 g
Calcium carbonate0.75 g
Boil, cool, add:
572
Columbia 5% Sheep Blood Agar (BBL)
Columbia Agar (BBL) +5% Sheep blood
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
859
Chocolate II Agar (BBL)
Pancreatic Digest of Casein7.5 g
Selected Meat Peptone7.5 g
Corn Starch1.0 g
Dipotassium Phosphate4.0 g
Monopotassium Phosphate1.0 g
Sodium Chloride5.0 g
Hemoglobin10.0 g
IsoVitaleX Enrichment10.0 mL
Agar12.0 g
Distilled water1000 mL
IsoVitaleXTM Enrichment
Vitamin B120.01 g
L-Glutamine10.0 g
Adenine0.03 g
/p/-Aminobenzoic Acid0.013 g
Nicotinamide Adenine Dinucleotide0.25 g
Thiamine Pyrophosphate0.1 g
Ferric Nitrate0.02 g
Thiamine Hydrochloride0.003 g
L-Cysteine Hydrochloride25.9 g
L-Cystine1.1 g
Dextrose100.0 g
Distilled water1000 mL

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