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Bacteria



BCRC Number: 14514
Organism: Clostridium sporogenes
Synonym: Clostridium putrificum
Author: (Metchnikoff)Bergey et al.
History: << ATCC << L. DS. Smith VPI 4440-1 << A. Prevot 2318
Other Collection No.: ATCC 25784 ;DSM 1734 ;NCIMB 10677
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B3234
Judicial Commission of the International Committee on Systematic Bacteriology 1999. Rejection of Clostridium putrificum and conservation of Clostridium botulinum and Clostridium sporogenes - opinion 69. Int. J. Syst. Bacteriol. 49: 339.
Keyword
Clostridium putrificum,Clostridium botulinum, Clostridium sporogenes
 

Medium



38
BEEF LIVER MEDIUM for Anaerobes
Tap water1.0 L
Peptone10.0 g
K2HPO41.0 g
Beef liver (cut in small pieces)500.0 g
Soak beef liver with 1 L tap water overnight in refrigerator. Skim fat off top. Sterilize at 121°C for 10 minutes. Filter through cheesecloth. Save meat. Add peptone and K2HPO4. Adjust pH to 8.0. Filter through filter paper and make up volume to 1.0 L with tap water. Boil broth and cool under 100% N2.Place a small amount of CaCO3 in each tube, add meat to a depth of 1/2 inch, cover with broth to a total depth of 2 inches.

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