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Bacteria



BCRC Number: 14513 Add To Bookmark
Organism: Hathewaya limosa
Synonym: Clostridium limosum
Author: (André) Lawson and Rainey
History: << ATCC (Clostridium limosum) << W. E. C. Moore VPI 2700 << A. Prévot A46
Other Collection No.: ATCC 25620 ;DSM 1400 ;NCIMB 10638
Source: mud, Africa
Characterization:
Taxonomy : Rename Clostridiium sp. to Hathewaya sp.--B000421,B000422;
Others: 1. Rename as DSM 1400.
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1805
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References



B000421
Lawson, P. A., and F. A. Rainey. 2016. Proposal to restrict the genus Clostridium Prazmowski to Clostridium butyricum and related species. Int. J. Syst. Evol. Microbiol. 66: 1009-1016.
B000422
Oren, A., and G. M. Garrity. 2016. Notification list. Notification that new names and new combinations, and new taxonomic opinions have appeared in volume 66, part 2 of the IJSEM. Int. J. Syst. Evol. Microbiol. 66: 1916-1919.
B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B1805
Prévot, A. R. 1948. Étude des bactéries anaérobies d'Afrique occidentale française (Sénégal, Guinée, Côte d'Ivoire). Annales de l'Institut Pasteur (Paris) 74: 157-170.

Medium



38
BEEF LIVER MEDIUM for Anaerobes
Tap water1.0 L
Peptone10.0 g
K2HPO41.0 g
Beef liver (cut in small pieces)500.0 g
Soak beef liver with 1 L tap water overnight in refrigerator. Skim fat off top. Sterilize at 121°C for 10 minutes. Filter through cheesecloth. Save meat. Add peptone and K2HPO4. Adjust pH to 8.0. Filter through filter paper and make up volume to 1.0 L with tap water. Boil broth and cool under 100% N2.Place a small amount of CaCO3 in each tube, add meat to a depth of 1/2 inch, cover with broth to a total depth of 2 inches.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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