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Bacteria



BCRC Number: 14513
Organism: Clostridium limosum
Author: Andre
History: << ATCC << W. E. C. Moore VPI 2700 << A. Prevot A46
Other Collection No.: ATCC 25620 ;ATCC 25760 ;DSM 1400 ;NCIMB 10638
Source: mud, Africa
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1805
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
B1805
Prvot, A. R. 1947. Etude des bactries anarobies dfrique occidentale franaise. Ann. Inst. Pasteur (Paris) 74: 157-170.
 

Medium



38
BEEF LIVER MEDIUM for Anaerobes
Tap water1.0 L
Peptone10.0 g
K2HPO41.0 g
Beef liver (cut in small pieces)500.0 g
Soak beef liver with 1 L tap water overnight in refrigerator. Skim fat off top. Sterilize at 121°C for 10 minutes. Filter through cheesecloth. Save meat. Add peptone and K2HPO4. Adjust pH to 8.0. Filter through filter paper and make up volume to 1.0 L with tap water. Boil broth and cool under 100% N2.Place a small amount of CaCO3 in each tube, add meat to a depth of 1/2 inch, cover with broth to a total depth of 2 inches.

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