Bacteria



BCRC Number: 14485
Organism: Clostridium sticklandii
Author: Stadtman and McClung
History: << DSMZ << A. C. Schwartz << T. C. Stadtman HF
Other Collection No.: DSM 519; ATCC 12662; NCIB 10654
Source: mud water
Category: anaerobe
Growth Conditions: 37°C 3 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
 

Medium



53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
63
COLSTRIDIUM STICKLANDII MEDIUM
KH2PO40.68 g
Distilled water1.0 L
Tryptone20.0 g
Yeast extract10.0 g
K2HPO41.04 g
Adjust pH to 7.0, After sterilization add 0.15 g Na2S·9H2O, sterilized separately.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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