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BCRC Number: 14477 Add To Bookmark
Organism: Prevotella intermedia
Synonym: Bacteroides melaninogenicus subsp. intermedius
Author: (Holdeman and Moore) Shah and Collins
History: << ATCC << L. V. Holdeman VPI 4197 (Bacteroides melaninogenicus subsp. intermedius) << S. Finegold B422
Other Collection No.: ATCC 25611; JCM 7365
Source: empyema
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1,B1397
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Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.
Shah, H. N., and D. M. Collins. 1990. Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int. J. Syst. Bacteriol. 40: 205-208.


Ground beef (fat free)500.0 g
1N NaOH25.0 mL
Distilled water1.0 L
Peptone30.0 g
Yeast extract5.0 g
L-cysteine·HCl0.5 g
K2HPO45.0 g
0.025% Resazurin solution4.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust.
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g


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