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BCRC Number: 14468 Add To Bookmark
Organism: Clostridium aceticum
Author: Gottschalk and Braun
History: << DSMZ << M. Braun << H. A. Barker << K. T. Wieringa
Other Collection No.: DSM 1496 ;ATCC 35044
Source: mud
Category: anaerobe
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1379
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Gottschalk, G., and M. Braun. 1981. Revival of the name Clostridium aceticum. Int. J. Syst. Bacteriol. 31: 476.


Resazurin1.0 mg
NaHCO310.0 g
NH4Cl1.0 g
KH2PO40.33 g
K2HPO40.45 g
MgSO4·7H2O0.1 g
Trace element solution (see below)20.0 ml
Vitamin solution (see below)20.0 ml
Yeast extract2.0 g
Fructose10.0 g
Cysteine·HCl0.5 g
Na2S·9H2O0.5 g
Distilled water1.0 L
Dissolve ingredients except NaHCO3, fructose, cysteine and sodium sulfide, bring to the boil for few minutes and cool to room temperature under N2 + CO2 (80% + 20%) gas mixture. Add NaHCO3 (solid) and equilibrate the medium with the gas until a pH of 7.4 is reached. Then distribute and autoclave under the same gas. Before use adjust the pH to 8.2 by adding sterile anaerobic Na2CO3 solution (0.25 ml of 5% Na2CO per 10 ml medium) and add fructose, cysteine and sodium sulfide from anaerobic sterile stock solutions.
Trace element solution:
Nitrilotriacetic acid1.5 g
MgSO4·7H2O3.0 g
MnSO4·2H2O0.5 g
NaCl1.0 g
FeSO4·7H2O0.1 g
CoSO4·7H2O0.18 g
CaCl2·2H2O0.1 g
ZnSO4·7H2O0.18 g
CuSO4·5H2O0.01 g
KAl(SO4)2·12H2O0.02 g
H3BO30.01 g
Na2MoO4·2H2O0.01 g
NiCl2·6H2O30.0 mg
Na2SeO3·5H2O0.3 mg
Na2WO4 x 2 H2O0.4 mg
Distilled water1.0 L
First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final pH to 7.0 with KOH.

Vitamin solution:
Biotin2.0 mg
Folic acid2.0 mg
Pyridoxin·HCl10.0 mg
Thiamine·HCl5.0 mg
Riboflavin5.0 mg
Nicotinic acid5.0 mg
D-Calcium pantothenate5.0 mg
Vitamin B120.1 mg
p-Aminobenzoic acid5.0 mg
Lipoic acid5.0 mg
Distilled water1.0 L


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