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Bacteria



BCRC Number: 14442 Add To Bookmark
Organism: Prevotella veroralis
Author: (Watabe et al.) Shah and Collins
History: << ATCC << J. Watabe VPI D22-7 << T. Mitsuoka << W. E. C. Moore
Other Collection No.: ATCC 33779
Source: human oral cavity
Category: anaerobe
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1369
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References



B1369
Watabe, J., Y. Benno, and T. Mitsuoka. 1983. Taxonomic study of Bacteroides oralis and related organisms and proposal of Bacteroides veroralis sp. nov. Int. J. Syst. Bacteriol. 33: 57-64.

Medium



190
CHOPPED MEAT MEDIUM
Ground beef (fat free)500.0 g
1N NaOH25.0 mL
Distilled water1.0 L
Peptone30.0 g
Yeast extract5.0 g
L-cysteine·HCl0.5 g
K2HPO45.0 g
0.025% Resazurin solution4.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust.
53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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