Bacteria



BCRC Number: 14430 Add To Bookmark
Organism: Prevotella denticola
Author: (Shah and Collins) Shah and Collins emend. Wu et al.
History: << ATCC << L. V. Holdeman VPI 10043 (Bacteroides melaninogenicus subsp. melaninogenicus) << D. W. Lambe, Jr. strain Emory 1284-74B
Other Collection No.: ATCC 33185
Source: maxillary atrium
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
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References



Medium



190
CHOPPED MEAT MEDIUM
Ground beef (fat free)500.0 g
1N NaOH25.0 mL
Distilled water1.0 L
Peptone30.0 g
Yeast extract5.0 g
L-cysteine·HCl0.5 g
K2HPO45.0 g
0.025% Resazurin solution4.0 ml
Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH and bring to a boil while stirring. Cool to room temperature, skim fat off surface, filter and retaining both meat particles and filtrate. Add sufficient distilled water to filtrate to restore 1.0 L original volume. To the filtrate add peptone, yeast extract, K2HPO4 and resazurin solution. Boil, then cool under O2-free nitrogen and 3% hydrogen, and add 0.5 g L-cysteine·HCl. Adjust pH to 7.0. Dispense 4-5 parts fluid to 1 part meat particles per test tube. Cap with rubber stoppers under N2 and H2 and autoclave in press for 15 minutes under fast exhaust.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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