BCRC Number: 14423
Organism: Moorella thermoacetica
Author: (Fontaine et al.) Collins et al.
History: << DSMZ << J. R. Andreesen << L. G. Ljungdahl << H. G. Wood << H. A. Barker << F. Fontaine
Other Collection No.: DSM 521;ATCC 35608
Source: horse feces
Category: anaerobe
Growth Conditions: 55°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.


Prepare solution A anaerobically under 100% CO2. Distribute and autoclave under CO2. Autoclave separatly solutions B, C, E and F under 100% N2, and solution D under CO2 gas atmosphere. After sterilization combine all solutions aseptically. Adjust pH of the complete medium to 6.9 if necessary.
Solution A:
Fe(NH4)2(SO4)20.04 g
Na2MoO4·2H2O2.4 mg
Na2SeO30.15 mg
Tryptone5.0 g
Yeast extract5.0 g
(NH4)2SO40.5 g
MgSO4·7H2O0.1 g
Resazurin1.0 mg
Distilled water800 mL

Solution B:
K2HPO47.0 g
KH2PO44.5 g
Distilled water50 mL

Solution C:
Glucose18.0 g
Distilled water50 mL

Solution D:
NaHCO310.0 g
Distilled water100 mL

Solution E:
Cysteine HCl0.3 g
Distilled water10 mL

Solution F:
Na2S·9H2O0.3 g
Distilled water10 mL

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