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Bacteria



BCRC Number: 14421 Add To Bookmark
Organism: Centipeda flueggeii
Synonym: Selenomonas flueggeii
Author: (Moore et al.) Heng and Kittelmann
History: << ATCC << L. V. H. Moore VPI E4M-28B << W. E. C. Moore
Other Collection No.: ATCC 43531
Source: human teeth
Characterization:
Taxonomy : Rename to Genus Centipeda. --B000497,B000505;
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1353,B000497,B000505
Shipping/Field/Price:
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References



B000497
Heng, Y. C. and S. Kittelmann. 2026. Taxonomic reclassification and expansion of the family Selenomonadaceae. Int. J. Syst. Evol. Microbiol. 76: 007178.
B000505
Oren, A. and M. Göker. 2026. Notification list. Notification that new names and new combinations have appeared in volume 76, part 5 of the IJSEM. Int J Syst Evol Microbiol 76: 7223.
B1353
Moore, L. V. H., J. L. Johnson, and W. E. C. Moore. 1987. Selenomonas noxia sp. nov., Selenomonas flueggei sp. nov., Selenomonas infelix sp. nov., Selenomonas dianae sp. nov. and Selenomonas artemidis sp. nov., from the human gingival crevice. Int. J. Syst. Bacteriol. 37 : 271–280.

Medium



229
MODIFIED CHOPPED MEAT MEDIUM
0.025% Resazurin solution4.0 mL
1N NaOH25.0 mL
Agar (if necessary)20.0 g
Cysteine·HCl0.5 g
Distilled water1.0 L
Ground beef (fat free)500.0 g
Hemin solution (see below)10.0 mL
K2HPO45.0 g
Trypticase peptone30.0 g
Vitamin K1 solution (see below)0.2 mL
Yeast extract5.0 g
Mix meat, water and NaOH and bring to a boil with stirring. Cool to room temperature, skim fat from surface, and filter, retaining both meat particles and filtrate. To filtrate, add sufficient distilled water to restore volumn to 1.0 L. To this filtrate, add trypticase peptone, yeast extract, K2HPO4, resazurin solution and agar (if necessary). Boil and cool medium under 80% N2, 10% CO2, and 10% H2, then add Cysteine-HCl, Hemin solution and Vitamin K1 solution. Adjust pH to 7.0. Anaerobically dispense 7 mL into tubes containing meat particles (1 part meat to 5 parts fluid) under the same gas phase.
Hemin solution:
Dissolve 50 mg hemin in 1mL 1N NaOH, make up to 100 mL with distilled water. Store refrigerated.

Vitamin K1 solution:
Dissolve 0.15 mL of Vitamin K1 in 30 mL of 95% ethanol and filter sterile. Keep Vitamin K1 solution under refrigeration. Store in brown bottle.

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