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Bacteria



BCRC Number: 14417 Add To Bookmark
Organism: Porphyromonas gingivalis
Author: (Coykendall et al.) Shah and Collins
History: << DSMZ << ATCC << A. I. Coykendall << J. Slots 2561
Other Collection No.: ATCC 33277; DSM 20709
Source: human gingival sulcus
Category: anaerobe
Growth Conditions: 37°C 7 day(s)
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1356
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References



B1356
Crykendall, A. L., F. S. Kaczmarek, and J. Siots. 1980. Genetic heterogeneity in B. asaccharolyticus (Holdeman and Moore 1980) Finegold and Barnes 1977 (Approved List. 1980) and proposal of Bacteroides gingivalis sp. nov. and Bacteroides macacae (Slots and Genco) comb. nov. Int. J. Syst. Bacteriol. 30 : 559–564.

Medium



1036
CDC anaerobic Blood Agar
Pancreatic Digest of Casein15.0 g
Soy peptone5.0 g
NaCl5.0 g
Yeast extract5.0 g
L-Cystine0.4 g
NaOH4.0 mL
Hemin(4.0% solution)5.0 mL
Vitamin K1(0.1% solution)1.0 mL
Agar15.0 g
Distilled water1.0 L
Defibrinated sheep blood50.0 mL
After autoclaved, when the medium cooling down to room temperature 45 degree celsius, added the defibrinated sheep blood.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
950
Supplemented Tryptic Soy Broth or Agar
Tryptic Soy Broth30.0 g
Yeast Extract5.0 g
L-cysteine hydrochloride0.5 g
Hemin Stock(see below)1.0 mL
Vitamin K1 Stock*(5 mg/mL)0.2 mL
Agar (If required)15.0 g
DI Water1000 mL
Autoclave at 121℃.Wrap each bottle in aluminum foil or keep bottle in dark as hemin and vitamin K are light sensitive and will be degraded. Supplemented TSB can be pre-reduced and pre-warmed by incubating a bottle of fresh media in the anaerobic chamber overnight before using it. *Menadione (vitamin K3) can be used at a final concentration of 1.0 mcg/mL instead of vitamin K1.
Hemin Stock Solution
Hemin0.5 g
K2HPO41.74 g
DI Water100 mL
Boil the above ingredients in a large flask. Store in the dark. *Note: Hemin will have a final concentration of 5 mcg/mL.

Vitamin K1 will have a final concentration of 1.0 mcg/ml.

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