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Actinomycetes



BCRC Number: 13698 Add To Bookmark
Organism: Gordonia sputi
Synonym: Gordona sputi;Rhodococcus sputi
Author: (Tsukamura) Stackebrandt et al.
History: << DSM << JCM <, KCC << M. Tsukamura, 3884, Nagura 8539
Other Collection No.: ATCC 29627 ;DSM 43896 ;JCM 3228
Source: sputum of patient with pulmonary disease--B940, B941
Growth Conditions: 37°C
Biosafety Level: 2
Type Strain: YES
    Reference: B940,B941
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References



B940
Tsukamura, M. 1978. Numerical classification of Rhodococcus (formerly Gordona) organisms recently isolated from sputa of patients: description of Rhodococcus sputi Tsukamura sp. nov. Int. J. Syst. Bacteriol. 28: 169-181.
B941
Tsukamura, M., and I. Yano. 1985. Rhodococcus sputi sp. nov., nom. rev., and Rhodococcus aurantiacus sp. nov., nom. rev. Int. J. Syst. Bacteriol. 35: 364-368.

Medium



77
GLYCEROL-SOIL MEDIUM
Peptone5.0g
Beef extract3.0g
Glycelor20.0g
Soil extract150.0ml
Tap water850.0ml
Agar15.0g
Adjust pH to 7.0. Soil extract is prepared by sieving air dried garden soil through a coarse sieve and autoclaving 400g with 960ml of tap water at 121oC foe 1 hr. After the mixture is cool and settled, the supernatant is carefully decanted, filtered through paper, autoclav

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