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Bacteria



BCRC Number: 11847
Organism: Bifidobacterium longum subsp. longum
Synonymy: Bifidobacterium longum
Author: Reuter
History: << DSMZ << G. Reuter E194b (Variant a)
Other Collection No.: ATCC 15707; DSM 20219
Source: adult intestine
Others: Taxonomy--B4159
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



 

Medium



252
LACTOBACILLI MRS BROTH with 0.05% Cysteine
Ammonium citrate2.0g
Beef extract10.0g
CH3COONa5.0g
Cysteine0.5g
Dextrose20.0g
Distilled water1.0L
K2HPO42.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
Proteose peptone No.310.0g
Tween 801.0g
Yeast extract5.0g
1.After preparation, distribute tubes under N2 and autoclave.
2.If needed, add agar powder 15.0 gram per liter.
53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.

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