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Bacteria



BCRC Number: 11845 Add To Bookmark
Organism: Bifidobacterium bifidum
Synonym:
Author: (Tissier) Orla-Jensen
History: << DSMZ << T.Mitsuoka << Tissier, Ti.
Other Collection No.: ATCC 29521; DSM 20456
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



Medium



252
LACTOBACILLI MRS BROTH with 0.05% Cysteine
Ammonium citrate2.0g
Beef extract10.0g
CH3COONa5.0g
Cysteine0.5g
Dextrose20.0g
Distilled water1.0L
K2HPO42.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
Proteose peptone No.310.0g
Tween 801.0g
Yeast extract5.0g
1.After preparation, distribute tubes under N2 and autoclave.
2.If needed, add agar powder 15.0 gram per liter.
53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g

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