Bacteria



BCRC Number: 11509 Add To Bookmark
Organism: Escherichia coli
Author: (Migula) Castellani and Chalmers
History: << YMMC << ATCC << FDA strain Seattle 1946 << F. Schoenknecht
Other Collection No.: ATCC 25922; CIP 76.24; DSM 1103; JCM 5491; NCIMB 12210
Source: clinical isolate
Characterization:
Test : γ-hemolysis
Others: quality control of MIC results by broth or by agar dilution;quality control of antimicrobial disk susceptibility test--B187,B206;control strain for BBL Minitek Identification System
Growth Conditions: 37°C 2 day(s)
Biosafety Level: 2
Oxygen Requirement: Aerobic
Shipping/Field/Price:
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References



Medium



66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.
675
Trypticase Soy Broth (BBL 211768) or Trypticase Soy Agar
Pancreatic digest of casein 17 g
Papaic digest of soybean3 g
Sodium chloride 5 g
Dipotassium phosphate 2.5 g
Dextrose2.5 g
Distilled water 1 L
Warm gently until solution is complete. Sterilize by autoclaving at 121°C for 15 minutes. If needed, add 15 g agar per liter medium.

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