Bacteria



BCRC Number: 11258 Add To Bookmark
Organism: Clostridium sporogenes
Author: (Metchnikoff) Bergey et al.
History: << ATCC << NCTC << M. Robertson SR5
Other Collection No.: ATCC 19404; DSM 1664; NCIMB 532; NCTC 532
Source: gas gangrene
Others: 1.sterility testing--B314
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
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References



Medium



38
BEEF LIVER MEDIUM for Anaerobes
Tap water1.0 L
Peptone10.0 g
K2HPO41.0 g
Beef liver (cut in small pieces)500.0 g
Soak beef liver with 1 L tap water overnight in refrigerator. Skim fat off top. Sterilize at 121°C for 10 minutes. Filter through cheesecloth. Save meat. Add peptone and K2HPO4. Adjust pH to 8.0. Filter through filter paper and make up volume to 1.0 L with tap water. Boil broth and cool under 100% N2.Place a small amount of CaCO3 in each tube, add meat to a depth of 1/2 inch, cover with broth to a total depth of 2 inches.
53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
572
Columbia 5% Sheep Blood Agar (BBL)
Columbia Agar (BBL) +5% Sheep blood
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
859
Chocolate II Agar (BBL)
Pancreatic Digest of Casein7.5 g
Selected Meat Peptone7.5 g
Corn Starch1.0 g
Dipotassium Phosphate4.0 g
Monopotassium Phosphate1.0 g
Sodium Chloride5.0 g
Hemoglobin10.0 g
IsoVitaleX Enrichment10.0 mL
Agar12.0 g
Distilled water1000 mL
IsoVitaleXTM Enrichment
Vitamin B120.01 g
L-Glutamine10.0 g
Adenine0.03 g
/p/-Aminobenzoic Acid0.013 g
Nicotinamide Adenine Dinucleotide0.25 g
Thiamine Pyrophosphate0.1 g
Ferric Nitrate0.02 g
Thiamine Hydrochloride0.003 g
L-Cysteine Hydrochloride25.9 g
L-Cystine1.1 g
Dextrose100.0 g
Distilled water1000 mL

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