Bacteria



BCRC Number: 11051 Add To Bookmark
Organism: Lactobacillus delbrueckii subsp. lactis
Synonym: Lactobacillus lactis; Lactobacillus leichmanii
Author: (Orla-Jensen) Weiss et al.
History: << ATCC << R. P. Tittsler 313 (Lactobacillus leichmannii) << E. McCoy Ld5 (Lactobacillus delbrueckii)
Other Collection No.: ATCC 7830; BCRC 14067; CSCC 3100; DSM 20355; IAM 12066; IFO 3376; JCM 1557; NCDO 302; NCIMB 8118
Characterization:
Assay : cobalamins--B290, B291;
vitamin B12--B168, B287, B288;
Taxonomy : Reclassification of the genus Lactobacillus--B000222;
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Facultatively anaerobic
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References



B000222
Zheng, J., S. Wittouck, E. Salvetti, C. M. A. P. Franz, H.M.B. Harris, P. Mattarelli, P. W. O'Toole, B. Pot, P. Vandamme, J. Walter, K. Watanabe, S. Wuyts, G. E. Felis, M. G. Gänzle, and S. Lebeer. 2020. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int. J. Syst. Evol. Microbiol. 70: 2782-2858.
B168
Skeggs, H. R. 1963. Lactobacillus leichmannii assay for vitamin B12. In Kavanagh, F. (ed.), Analytical Microbiology. pp. 551-565. Academic Press, Inc., New York.
B287
Hoffmann, C. E. et al. 1948. Response of Lactobacillus leichmannii 313 to the antipernicious anemia factor. J. Biol. Chem. 176: 1465-1466.
B288
U. S. P. 1980. Vitamin B12 activity assay. In The United States Pharmacopeia, 20th rev. pp. 903-905. United States Pharmocopeial Convention, Inc., Maryland.
B290
1963. Vitamin B12 and congeners. In Kavanagh, F. (ed.). Analytical Microbiology. pp. 543-544 and 546-547. Academic Press, Inc., New York.
B291
A. O. A. C. 1980. Cobalamin (Vitamin B12 activity) (22) 43.135-43.141. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.

Medium



16
LACTOBACILLI MRS BROTH (DIFCO 0881)
Proteose peptone No.310.0g
Beef extract10.0g
Yeast extract5.0g
Dextrose20.0g
Tween 801.0g
Ammonium citrate2.0g
CH3COONa5.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
K2HPO42.0g
Distilled water1.0L
Adjust pH to 6.2-6.5. 1.If needed, add agar powder 15.0 gram per liter.

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