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Bacteria



BCRC Number: 10940
Organism: Lacticaseibacillus rhamnosus
Synonym: Lactobacillus rhamnosus; Lactobacillus casei subsp. rhamnosus
Author: (Hansen) Collins et al.
History: << ATCC (Lactobacillus rhamnosus) << F. M. Strong (Lactobacillus casei)
Other Collection No.: ATCC 7469; CCM 1825; DSM 20021; NCDO 243; NCIB 6375; NCTC 6375
Characterization:
Assay : 6-mercaptopurine--B257;
azathioprine
arginine--B256, B256;
pyridoxal--B256;
biotin--B256;
glutamic acid--B256;
nicotinic acid--B256;
pantothenic acid--B256;
riboflavin--B255;
folic acid--B254;
Taxonomy : Reclassification of the genus Lactobacillus--B000222;
Others: respiratory physiology
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Facultatively anaerobic
Type Strain: YES
    Reference: B253,B886
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References



B000222
Zheng, J., S. Wittouck, E. Salvetti, C. M. A. P. Franz, H.M.B. Harris, P. Mattarelli, P. W. O'Toole, B. Pot, P. Vandamme, J. Walter, K. Watanabe, S. Wuyts, G. E. Felis, M. G. Gänzle, and S. Lebeer. 2020. A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae. Int. J. Syst. Evol. Microbiol. 70: 2782-2858.
B253
Hansen, P. A. 1968. Type strains of Lactobacillus species. In American Type Culture Collection. p. 76. Rockville, Maryland. U. S. A.
B254
Teply, L. J., and C. A. Elvehjem. 1945. The titrimetric determination of "Lactobacillus casei factor" and "folic acid". J. Biol. Chem. 157: 303-309.
B255
1969. Difco manual of dehydrated culture media and reagents for microbiological and clinical laboratory procedures. In Difco Laboratories, Inc., 9th ed., Detroit, Michigan, U. S. A.
B256
Girdwood, R. H. 1963. The microbiological assay of vitamins and amino acids. J. Med. Lab. Technol. 20: 26-33.
B257
Harber, M. J., and J. L. Maddocks. 1973. Microbiological assay for purine analogues with Lactobacillus casei. J. Gen. Microbiol. 79: 351-353.
B886
Collins, M. D., B. A. Phillips, and P. Zanoni. 1989. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans and Lactobacillus rhamnosus sp. nov., comb. nov. Int. J. Syst. Bacteriol. 39 : 105–108.
 

Medium



16
LACTOBACILLI MRS BROTH (DIFCO 0881)
Proteose peptone No.310.0g
Beef extract10.0g
Yeast extract5.0g
Dextrose20.0g
Tween 801.0g
Ammonium citrate2.0g
CH3COONa5.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
K2HPO42.0g
Distilled water1.0L
Adjust pH to 6.2-6.5. 1.If needed, add agar powder 15.0 gram per liter.

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