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Bacteria



BCRC Number: 10699
Organism: Lactobacillus delbrueckii subsp. lactis
Synonymy: Lactobacillus lactis
Author: (Orla-Jensen) Weiss et al.
History: << ATCC << R. P. Tittsler 326 (Lactobacillus leichmannii) << E. B. Fred F59
Other Collection No.: ATCC 4797 ;CCM 2344 ;DSM 20076 ;IFO 3073 ;NCDO 299 ;NCIB 7854
Characterization:
Assay of : vitamin B12--B168;
cobalamins--B167;
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Facultatively anaerobic
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References



B167
1963. Lactobacillus leichmannii assay for cobalamins. In Kavanagh, F. (ed.). Analytical Microbiology. pp. 543-547. Academic Press, Inc., New York.
B168
Skeggs, H. R. 1963. Lactobacillus leichmannii assay for vitamin B12. In Kavanagh, F. (ed.), Analytical Microbiology. pp. 551-565. Academic Press, Inc., New York.
 

Medium



16
LACTOBACILLI MRS BROTH (DIFCO 0881)
Proteose peptone No.310.0g
Beef extract10.0g
Yeast extract5.0g
Dextrose20.0g
Tween 801.0g
Ammonium citrate2.0g
CH3COONa5.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
K2HPO42.0g
Distilled water1.0L
Adjust pH to 6.2-6.5. 1.If needed, add agar powder 15.0 gram per liter.

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