Bacteria



BCRC Number: 10681 Add To Bookmark
Organism: Fusobacterium nucleatum subsp. nucleatum
Author: Knorr
History: << ATCC << W. E. C. Moore VPI 4355 << A. Prevot 1612A
Other Collection No.: ATCC 25586; CCUG 32989; CIP 101130 ;JCM 8532; LMG 13131 ;DSM 15643
Source: Cervico-facial lesion
Others: 1.lysine metabolism
2.(2016-03-04)經測試後,於72號及859號培養基生長良好。建議用此培養。
Category: anaerobe
Growth Conditions: 37°C
Biosafety Level: 2
Oxygen Requirement: Anaerobic
Type Strain: YES
    Reference: B1
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References



B1
Skerman, V. B. D., V. McGowan, and P. H. A. Sneath. 1980. Approved lists of bacterial names. Int. J. Syst. Bacteriol. 30 : 225–420.

Medium



53
REINFORCED CLOSTRIDIAL MEDIUM (OXOID CM149)
'Lab-lemco' powder10.0 g
Peptone10.0 g
Cysteine·HCl0.5 g
NaCl5.0 g
CH3COONa3.0 g
Soluble starch1.0 g
Glucose5.0 g
Yeast extract3.0 g
Agar0.5 g
Distilled water1.0 L
Adjust pH to 6.8. Bring to the boil to dissolve completely. Autoclave at 121°C/15 min.
72
TRYPTIC SOY AGAR with 5% Defibrinated Sheep Blood
Agar15.0 g
Defibrinated Sheep Blood50.0 mL
Distilled water1.0 L
NaCl5.0 g
Soytone5.0 g
Tryptone15.0 g
859
Chocolate II Agar (BBL)
Pancreatic Digest of Casein7.5 g
Selected Meat Peptone7.5 g
Corn Starch1.0 g
Dipotassium Phosphate4.0 g
Monopotassium Phosphate1.0 g
Sodium Chloride5.0 g
Hemoglobin10.0 g
IsoVitaleX Enrichment10.0 mL
Agar12.0 g
Distilled water1000 mL
IsoVitaleXTM Enrichment
Vitamin B120.01 g
L-Glutamine10.0 g
Adenine0.03 g
/p/-Aminobenzoic Acid0.013 g
Nicotinamide Adenine Dinucleotide0.25 g
Thiamine Pyrophosphate0.1 g
Ferric Nitrate0.02 g
Thiamine Hydrochloride0.003 g
L-Cysteine Hydrochloride25.9 g
L-Cystine1.1 g
Dextrose100.0 g
Distilled water1000 mL

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