Bacteria



BCRC Number: 10460 Add To Bookmark
Organism: Pseudomonas putida
Author: (Trevisan) Migula
History: << RMRC << ATCC << P. A. Williams mt-2 << G. D. Hegeman
Other Collection No.: ATCC 33015 ;JCM 6156
Others: TOL plasmid--B85,B86,B87
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
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References



B85
Williams, P. A., and K. Murray. 1974. Metabolism of benzoate and the methylbenzoates by Pseudomonas putida (arvilla) mt-2: evidence for the existence of a TOL plasmid. J. Bacteriol. 120: 416-423.
B86
Worsey, M. J., and P. A. Williams. 1975. Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. J. Bacteriol. 124: 7-13.
B87
Worsey, M. J., F. C. H. Franklin, and P. A. Williams. 1978. Regulation of the degradative pathway enzymes coded for by the TOL plasmid (pWWO) from Pseudomonas putida mt-2. J. Bacteriol. 134: 757-764.

Medium



592
Medium
Benzoate Medium For solid medium.
(NH4)2HPO43.0g
KH2PO41.2g
MgSO4 ·7H2O0.2g
Yeast extraet0.5g
Sodium benzoate (filter-sterilized)3.0g
Distilled water1.0L
NaCl5.0g
Agar Noble (Difco 0142)20.0g
Benzoate Medium For solid medium. : Combine all ingredients except sodium benzoate. Autoclave at 121℃ for 15 minutes, aseptically add filter-sterilized sodium benzoate.

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