Bacteria



BCRC Number: 10447
Organism: Bacillus subtilis subsp. spizizenii
Author: Nakamura et al.
History: << ATCC (Bacillus subtilis) << N. R. Smith 231 << K. Kellerman
Other Collection No.: ATCC 6633; NRRL B-765; BCRC 80045; CCM 1999; DSM 347; IAM 1069; IFO 3134; JCM 2499; NCIMB 8054; NCTC 10400
Characterization:
Assay of
streptomycin (in feeds)--B61
viomycin--B60

paromomycin
mitomycin
leucomycin

dihydrostreptomycin

actinomycin D
antibiotics--B1156
Production of subtilin--B62
Others: ◆sterility testing--B60,B61,B1155;
media testing--B1161
◆(2013-10-07)於 NA 平板上,菌落呈現片狀或乾片狀,流洗至液體培養液中不易均質。
◆(2013-10-07)若有配製懸浮液的需求時,建議使用 BCRC medium 66 培養,菌落長出後不致於會有片狀或乾片狀的情形產生,製成懸浮液才可呈現均勻化。
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
Shipping/Field/Price:
Freeze-Dried for all NTD$ 2500 Order this item


References



B1156
Code of Federal Regulations, Title 21. 1987.
B60
Laskin, A. I., and H. A. Lechevalier. (eds.). 1973. Handbook of Microbiology, Vol. III. Microbial Products. CRC Press. Inc., Cleveland, Ohio.
B61
A. O. A. C. 1980. Streptomycin (67), 42.287-42.294. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.
B62
Jansen, E. F., and D. J. Hirschmann. 1944. Subtilin-an antibacterial product of Bacillus subtilis culturing conditions and properties. Arch. Biochem. 4: 297-309.
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.
66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.

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