Bacteria



BCRC Number: 10447
Organism: Bacillus subtilis subsp. spizizenii
Author: Nakamura et al.
History: << ATCC (Bacillus subtilis) << N. R. Smith 231 << K. Kellerman
Other Collection No.: ATCC 6633; NRRL B-765; BCRC 80045; CCM 1999; DSM 347; IAM 1069; IFO 3134; JCM 2499; NCIMB 8054; NCTC 10400
Characterization:
Assay streptomycin (in feeds)--B61
viomycin--B60
paromomycin
mitomycin
leucomycin
dihydrostreptomycin
actinomycin D
antibiotics--B1156
Production subtilin--B62
Test sterility--B60,B61,B1155
media--B1161
Others:
Growth Conditions: 30°C
Biosafety Level: 1
Oxygen Requirement: Aerobic
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References



B1155
U. S. P. 1985. Biological test and assays. In The United States Pharmacopeia, 21st rev. pp. 1156-1157. United States Pharmacopeial Convention, Inc., Maryland.
B1156
Code of Federal Regulations, Title 21. 1987.
B1161
Brit. Pharmacopoeia. p. A106-A107. 1986.
B60
Laskin, A. I., and H. A. Lechevalier. (eds.). 1973. Handbook of Microbiology, Vol. III. Microbial Products. CRC Press. Inc., Cleveland, Ohio.
B61
A. O. A. C. 1980. Streptomycin (67), 42.287-42.294. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.
B62
Jansen, E. F., and D. J. Hirschmann. 1944. Subtilin-an antibacterial product of Bacillus subtilis culturing conditions and properties. Arch. Biochem. 4: 297-309.
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.
66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.

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