Bacteria



BCRC Number: 10447 Add To Bookmark
Organism: Bacillus spizizenii
Synonym: Bacillus subtilis subsp. spizizenii
Author: (Nakamura et al.) Dunlap et al.
History: << ATCC (Bacillus subtilis) << N. R. Smith 231 << K. Kellerman
Other Collection No.: ATCC 6633; NRRL B-765; BCRC 80045; CCM 1999; DSM 347; IAM 1069; IFO 3134; JCM 2499; NCIMB 8054; NCTC 10400
Characterization:
Assay : streptomycin (in feeds)--B61;
viomycin--B60;
paromomycin
mitomycin
leucomycin
dihydrostreptomycin
actinomycin D
antibiotics--B1156;
Production : subtilin--B62;
Test : sterility--B60,B61,B1155;
media--B1161;
Taxonomy : Subspecies rename to species--B000317,B000318;
Others: 1.Rename as Ref. no. B000317, B000318.
Growth Conditions: 30°C 1 day(s)
Biosafety Level: 1
Oxygen Requirement: Aerobic
Shipping/Field/Price:
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References



B000317
Dunlap, C. A., M. J. Bowman, and D. R. Zeigler. 2020. Promotion of Bacillus subtilis subsp. inaquosorum, Bacillus subtilis subsp. spizizenii and Bacillus subtilis subsp. stercoris to species status. Antonie Van Leeuwenhoek 113: 1-12.
B000318
Oren, A., and G. M. Garrity. 2020. Validation list no. 193. List of new names and new combinations previously effectively, but not validly, published. Int J Syst Evol Microbiol 70: 2960-2966.
B1155
U. S. P. 1985. Biological test and assays. In The United States Pharmacopeia, 21st rev. pp. 1156-1157. United States Pharmacopeial Convention, Inc., Maryland.
B1156
Code of Federal Regulations, Title 21. 1987.
B1161
Brit. Pharmacopoeia. p. A106-A107. 1986.
B60
Laskin, A. I., and H. A. Lechevalier. (eds.). 1973. Handbook of Microbiology, Vol. III. Microbial Products. CRC Press. Inc., Cleveland, Ohio.
B61
A. O. A. C. 1980. Streptomycin (67), 42.287-42.294. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.
B62
Jansen, E. F., and D. J. Hirschmann. 1944. Subtilin-an antibacterial product of Bacillus subtilis culturing conditions and properties. Arch. Biochem. 4: 297-309.

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.
66
TRYPTIC SOY BROTH OR TRYPTIC SOY AGAR (DIFCO 0369)
Tryptone15.0 g
Soytone5.0 g
NaCl5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.3.

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