Bacteria



BCRC Number: 10446
Organism: Bacillus cereus
Author: Frankland and Frankland
History: << TSCY << ATCC << FDA strain PCI 213 (Bacillus mycoides)
Other Collection No.: ATCC 11778 ;ATCC 9634 ;CCM 896 ;DSM 345 ;NCIMB 8012 ;NCTC 10320; KCTC 1012
Characterization:
Assay of minocycline--B59
tetracycline--B55
oxytetracycline (in feeds)--B58
chlortetracycline (in feeds)--B57
methacycline--B55
doxycycline--B55
demeclocycline--B55
rolitetracycline--B55
oxytetracycline--B55
chlortetracycline--B55
Growth Conditions: 30°C
Biosafety Level: 2
Oxygen Requirement: Aerobic
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References



B55
Dornbush, A. C., and A. Abbey. 1972. Microbiological assay of the tetracyclines. IV. Agar diffusion assays. In Kavanagh, F. (ed.). Analytical Microbiology, Vol. 2. pp. 374-379. Academic Press, Inc., New York.
B57
A. O. A. C. 1980. Chlortetracycline (CTC) hydrochloride (52), 42.211-42.214. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.
B58
A. O. A. C. 1980. Oxytetracycline(64), 42.272-42.277. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.
B59
Simon, H. J., and E. J. Yin. 1970. Microbioassay of antimicrobial agents. Appl. Microbiol. 19: 573-579.
 

Medium



3
NUTRIENT AGAR (DIFCO 0001)
Beef extract3.0 g
Peptone5.0 g
Agar15.0 g
Distilled water1.0 L
Adjust pH to 7.0.

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