Bacteria



BCRC Number: 10357
Organism: Lactobacillus plantarum subsp. plantarum
Author: (Orla-Jensen) Bringel et al.
History: << IAM << ATCC (Lactobacillus plantarum) << E. McCoy 17-5 (Lactobacillus arabinosus)
Other Collection No.: ATCC 8014; CCM 1904; DSM 20205; IAM 1041; IFO 3070; NCDO 82; NCIB 6376
Characterization:
Assay of : nicotinic acid
niacinamide
calcium pantothenate
niacin--B36, B37;
biotin--B35;
methionine--B34;
L(-)tryptophan--B33;
tryptophan--B32;
arginine
amino acids--B31;
Others: phage host
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Facultatively anaerobic
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References



B31
A. O. A. C. 1980. Amino acid (27), 43. 177-43. 187. In. Horwitz, W. (ed.), Official Methods of Analysis. A. O. A. C. Washington, D. C.
B32
Shankman, S. 1943. Amino acid nutrition of Lactobacillus arabinosus. J. Biol. Chem. 150: 305-310.
B33
Wooley, J. G., and W. H. Sebrell. 1945. Two microbiological methods for the determination of L(-)tryptophane in proteins and other complex substances. J. Biol. Chem. 157: 141-151.
B34
1950. USDA Misc. Publ. No. 696. Jan.
B35
Skeggs, H. R. 1963. Biotin. In Kavanagh, F. (ed.). Analytical Microbiology. pp. 421-428. Academic Press, Inc., New York.
B36
Hegsted, D. M., and C. E. Alvistur. 1952. Microbiologic assay for niacin. Anal. Chem. 24: 1635-1636.
B37
A. O. A. C. 1980. Niacin and niacinamide (nicotinic acid and nicotinamide). (24), 43.150-43.158. In Horwitz, W. (ed.). Official Methods of Analysis. A. O. A. C. Washington, D. C.
 

Medium



16
LACTOBACILLI MRS BROTH (DIFCO 0881)
Proteose peptone No.310.0g
Beef extract10.0g
Yeast extract5.0g
Dextrose20.0g
Tween 801.0g
Ammonium citrate2.0g
CH3COONa5.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
K2HPO42.0g
Distilled water1.0L
Adjust pH to 6.2-6.5. 1.If needed, add agar powder 15.0 gram per liter.

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