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Bacteria



BCRC Number: 80528 Add To Bookmark
Organism: Rhodovulum visakhapatnamense
Author: Srinivas et al.
History: << DSMZ << C. Sasikala, J. N. T. Univ., Inst. Sci. Technol., Hyderabad, India; JA 181 << P. A. Kumar
Other Collection No.: DSM 17937; ATCC BAA-1274; JCM 13531
Source: marine surface and tidal waters exposed to light
Isolated Info:
    Isolated location: India, eastern coast, Bay of Bengal, Ramakrishna beach at Visakhapatnam (17° 42' N 83° 18' E)
Others: 1.冷凍保存法:抗凍劑為 5% DMSO (最終濃度)。 2.冷凍小管只能解凍一次並且完全活化,不可重複冷凍解凍。 3.菌株復活:冷凍小管立即置於37-40℃水浴中,不時搖動,使冷凍菌液完全融解。回溶的菌液必須全部吸取至 10 ml 的新鮮培養基中進行活化培養。 4.特殊培養條件:厭氧和光照。 5.增殖培養的接種量需求:接種菌液的體積必須佔新鮮培養基的 20% 至 50%。
Growth Conditions: 25°C 7 day(s)
Biosafety Level: 1
Oxygen Requirement: Anaerobic
Type Strain: YES
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References



B4507
Srinivas, T. N. R., P. A. Kumar, C. Sasikala, C. Ramana, and J. F. Imhoff. 2007.. Rhodovulum visakhapatnamense sp. nov. Int. J. Syst. Evol. Microbiol. 57 : 1762-1764.

Medium



905
RHODOVULUM VISAKHUM MEDIUM
KH2PO40.50 g
MgCl2 ·7 H2O1.00 g
NaCl20.0 g
NH4Cl0.60 g
CaCl2· 2 H2O0.15 g
Yeast extract0.40 g
Sodium pyruvate 3.0 g
Distilled water1000 mL
Adjust to pH 6.8. Autoclave the medium and Trace element solution SL8 (see below) separately. Aseptically add 1.0 mL Trace element solution SL8 and 1.0 mL Vitamin B12 (2 mg/L concentration, filter sterilised) to the medium after autoclaving.
Trace element solution SL8
Na2· EDTA5.2 g
FeCl2·4 H2O1.5 g
ZnCl270.0 mg
MnCl2 · 4 H2O0.1 g
H3BO362.0 mg
CoCl2 · 6 H2O190.0 mg
CuCl2 · 2 H2O17.0 mg
NiCl2 · 6 H2O24.0 mg
Na2MoO4 · 2 H2O36.0 mg
Distilled water1000 mL
Adjust to pH 6.5.

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