Vector



BCRC Number: 41232 Add To Bookmark
Organism: YIp352
History: << ATCC<< A. Tzagoloff
Description: plasmid YIp352 can be used as a cloning vector for Saccharomyces cerevisiae and Escherichia coli and as a vector for selecting DNA sequences containing a replicon for S. cerevisiae. DNA sequences can be cloned in the lacZalpha region providing detection of E. coli transformants containing recombinant plasmids on the basis of their Lac- phenotype. Bacteria harboring recombinant plasmids make colorless colonies. Plasmid YIp325 contains several unique restriction sites in the lacZalpha region, downstream from the lacZ-initiation codon; the sequence with the cloning sites is the same as in M13mp18.
Other Collection No.: ATCC37675
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Reporter Gene: lacZ'
Vector Category: shuttle vector, cloning vector, integrating vector
Host Range: Saccharomyces cerevisiae Escherichia coli
Vector Type: plasmid
Replication Origin: pMB1
Select Marker: amp, pyrF(E. coli) URA3(yeast)
Promoter: lac
Shipped Host: Freeze-dried Escherichia coli RR1
Size(kb): 4.3
Multi Clone Site: Yes
Image / Description:   酵素複核電泳圖
 
Shipping/Field/Price:
Freeze-Dried for all NTD$ 3500 Order this item


References



P359
Hill, J. E., A. M. Myers, T. J. Koerner, and A. Tzagoloff. 1986. YeastE. coli shuttle vectors with multiple unique restriction sites. Yeast 2: 163-167.

Medium



290
YEAST TRYPTONE MEDIUM (LB Medium) with 100μg/mL Ampicillin
Ampicillin100.0 mg
Distilled water1.0 L
NaCl10.0 g
Tryptone10.0 g
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

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