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Bacteria



BCRC Number: 10940
Organism: Lactobacillus rhamnosus
Author: (Hansen) Collins et al.
History: << ATCC << F. M. Strong (Lactobacillus casei)
Other Collection No.: ATCC 7469; CCM 1825; DSM 20021; NCDO 243; NCIB 6375; NCTC 6375
Characterization:
Assay 6-mercaptopurine--B257
azathioprine
arginine--B256, B256
pyridoxal--B256
biotin--B256
glutamic acid--B256
nicotinic acid--B256
pantothenic acid--B256
riboflavin--B255
folic acid--B254
Others: respiratory physiology
Growth Conditions: 37°C
Biosafety Level: 1
Oxygen Requirement: Facultatively anaerobic
Type Strain: YES
    Reference: B253,B886
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References



B253
Hansen, P. A. 1968. Type strains of Lactobacillus species. In American Type Culture Collection. p. 76. Rockville, Maryland. U. S. A.
B254
Teply, L. J., and C. A. Elvehjem. 1945. The titrimetric determination of "Lactobacillus casei factor" and "folic acid". J. Biol. Chem. 157: 303-309.
B255
1969. Difco manual of dehydrated culture media and reagents for microbiological and clinical laboratory procedures. In Difco Laboratories, Inc., 9th ed., Detroit, Michigan, U. S. A.
B256
Girdwood, R. H. 1963. The microbiological assay of vitamins and amino acids. J. Med. Lab. Technol. 20: 26-33.
B257
Harber, M. J., and J. L. Maddocks. 1973. Microbiological assay for purine analogues with Lactobacillus casei. J. Gen. Microbiol. 79: 351-353.
B886
Collins, M. D., B. A. Phillips, and P. Zanoni. 1989. Deoxyribonucleic acid homology studies of Lactobacillus casei, Lactobacillus paracasei sp. nov., subsp. paracasei and subsp. tolerans and Lactobacillus rhamnosus sp. nov., comb. nov. Int. J. Syst. Bacteriol. 39 : 105–108.
 

Medium



16
LACTOBACILLI MRS BROTH (DIFCO 0881)
Proteose peptone No.310.0g
Beef extract10.0g
Yeast extract5.0g
Dextrose20.0g
Tween 801.0g
Ammonium citrate2.0g
CH3COONa5.0g
MgSO4·7H2O0.1g
MnSO4·H2O0.05g
K2HPO42.0g
Distilled water1.0L
Adjust pH to 6.2-6.5.

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