Cell Line



BCRC Number: 60015 Add To Bookmark
Name: GH3
Type: Rat pituitary tumor, somatotrophin secreting
Species: Rattus norvegicus (rat)
Strain Ethnicity: Wister-Furth
Age Stage: 7 months
Gender: Female
Tissue: Pituitary; tumor

Morphology: Epithelial and round cell cluster
Split Ratio: 1:2 to 1:4
Isoenzyme Analysis: MD; LD; NP; G6PD
Mycoplasma Test: Negative
Virus resistance: poliovirus 1
Virus susceptibility: herpes simplex; vesicular stomatitis (Indiana)

Incubation Temp(°C): 37°C
Incubation Condition: 5% CO2
Culture Medium: 82.5% Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate+ 15% horse serum + 2.5% fetal bovine serum
Growth Property: Adherent
SubCulture Procedure: trypsin-EDTA
Freezed Medium: 93% culture medium + 7% DMSO
Medium Renewal: every 2 to 3 days

Description: Established from a pituitary tumor carried in a 7-month-old female Wistar-Furth rat; the cells produce prolactin and growth hormone (somatotrophin); hydrocortisone stimulates the production of growth hormone and inhibits prolactin production.
Product Character: prolactin; growth hormone (somatotrophin)

Image / Description:   200倍視野下低細胞密度
  200倍視野下高細胞密度
 

Biosafety Level: 1
Reference: J. Cell Biol. 43: 432-441, 1969; ibid., 47: 61-70, 1970; In Vitro 6: 180-189, 1970
Shipping/Field/Price:
Frozen Vial for all NTD$ 7000 Order this item

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