Host



BCRC Number: 51957 Add To Bookmark
Organism: E. coli S17-1
History: << ATCC
Other Collection No.: 47055
Genotype: recA pro hsdR RP4-2-Tc::Mu-Km::Tn7
Description: Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified. RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome.
Growth Conditions: 37°C 1 day(s)
Biosafety Level: 1
Shipping/Field/Price:
Freeze-Dried for all NTD$ 3500 Order this item


References



H173
Parke D . . Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990.

Medium



90
LURIA-BERTANI (LB) MEDIUM
Tryptone10.0 g
NaCl10.0 g
Distilled water1.0 L
Yeast extract5.0 g
Adjust pH to 7.0-7.5.

Tags



Bioresource Collection and Research Center

331, Shih-Pin Rd., Hsinchu 300193, Taiwan

Phone: +886-3-5223191

E-mail: bcrcweb@firdi.org.tw

Copyright © 2018.BCRC All rights reserved.

web maintenance: +886-3-5223191 ext 572

Privacy Policy        Cookie